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| Registros recuperados: 13 | |
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| Sasamori,Márcio Hisayuki; Endres Júnior,Delio; Droste,Annette. |
| ABSTRACTCattleya intermediais an Atlantic Forest species endemic to Brazil that is classed as vulnerable on the list of threatened species. In this study, C. intermedia plantlets were micropropagated in an asymbiotic culture and the influence of different concentrations of sucrose (15, 30, 45 and 60 g L-1, plus a zero sucrose medium) and macronutrient salts (complete Murashige and Skoog (MS) medium and half MS medium (with half-strength macronutrients)) on survival and development of the plantlets was evaluated. In all treatments 100% plantlet survival was achieved. The integrated analysis of height of aerial part, number of leaves per plantlet, fresh mass, number of roots per plantlet and length of the longest root showed that the plantlets exhibited... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atlantic Forest; Carbohydrate; Conservation; In vitro culture; Orchids; Propagation. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300292 |
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| Körbes,Ana Paula; Droste,Annette. |
| Somatic embryogenesis is an efficient method for the production of target cells for soybean genetic transformation. However, this method still offers low percentages of plant regeneration, and perhaps is related to the maturation process and high morphological abnormalities of the matured embryos. This study aimed to identify a maturation medium that could contribute to the outcome of more efficient plant regeneration results. Embryogenic clusters, derived from cotyledons of immature seeds of the soybean cultivars Bragg and IAS5, were used as starting material for embryos development. Different maturation media were tested by using 6% maltose, 3% sucrose or 6% sucrose, combined with or without 25 g L-1 of the osmotic regulator polyethylene glycol... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; PEG-8000; Sucrose; Osmotic regulation. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000300003 |
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| Thewes,Márcia Regina; Endres Junior,Delio; Droste,Annette. |
| The genotoxicity of untreated and treated sewage from two municipal wastewater treatment plants (WTP BN and WTP SJN) in the municipality of Porto Alegre, in the southern Brazilian state of Rio Grande do Sul, was evaluated over a one-year period using the Tradescantia pallida var. purpurea (Trad-MCN) bioassay. Inflorescences of T. pallida var. purpurea were exposed to sewage samples in February (summer), April (autumn), July (winter) and October (spring) 2009, and the micronuclei (MCN) frequencies were estimated in each period. The high genotoxicity of untreated sewage from WTP BN in February and April was not observed in treated sewage, indicating the efficiency of treatment at this WTP. However, untreated and treated sewage samples from WTP SJN had high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Effluent; Micronucleus; Mutagenicity; Pollution; Trad-MCN. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400023 |
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| Wunder,Denise Angela; Droste,Annette; Windisch,Paulo Günter. |
| (Germinação de megásporos e desenvolvimento esporofítico inicial da filicínea Regnellidium diphylum (Marsileaceae) sob deficiências minerais). Regnellidium diphyllum Lindm. é uma filicínea heterosporada da família Marsileaceae, de distribuição restrita ao sul do Brasil e algumas localidades do Uruguai e da Argentina. A espécie se encontra na lista de espécies ameaçadas de extinção do Rio Grande do Sul, como vulnerável. No presente estudo, verificou-se o efeito da ausência de nutrientes minerais na germinação de megásporos e no desenvolvimento inicial de esporófitos de R. diphyllum in vitro. O efeito de soluções nutritivas de Meyer modificadas pela ausência de NH4NO3, NaCl, FeCl3·6H2O, MgSO4·7H2O, KH2PO4 ou CaCl2 foi analisado na germinação e na formação de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ecofisiologia; Macrófita aquática; Nutrição mineral; Pteridófitas; Reprodução. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042010000300013 |
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| Cassanego,Mara Betânia Brizola; Goldoni,Angélica; Heldt,Fágner Henrique; Osório,Daniela Montanari Migliavacca; Windisch,Paulo Günter; Droste,Annette. |
| Regnellidium diphyllum Lindm. is a heterosporous fern growing in wetlands and humid soils that are being converted to agricultural activities. Many products that are used in agriculture contain copper, resulting in surface and groundwater contamination. Germination and initial development tests were performed using Meyer's solution containing copper sulphate at concentrations of 0 (control), 1, 5, 10, 50 and 100 mg L-1. The experiment was conducted in a growth chamber at 25 ± 1ºC for 28 days, with a 12/12-hour light/dark cycle and a photon flux density of 100 µmol m-2 s-1. The lowest germination rate (6%) was observed at 100 mg L-1. Primary root growth was significantly reduced at > 10 mg L-1. Secondary leaves of sporophytes grown in concentrations >... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fern; Heavy metals; Pollution; Reproduction; Species conservation; Wetlands. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000100004 |
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| Endres Júnior,Delio; Sasamori,Márcio Hisayuki; Droste,Annette. |
| Anathallis adenochila is a small epiphytic orchid, endemic to the Atlantic Forest in the southern and southeastern regions of Brazil. Information on the species is scarce and limited to distribution and occurrence data, with no reports about its development. In the present study, plantlets were propagated in vitro, and the influence of different concentrations of macronutrient salts and sucrose on the survival and development of the species was assessed. The analysis were performed on complete Murashige and Skoog (MS) medium and half MS medium (with half-strength macronutrients) containing 10, 30 or 60 g L-1 of sucrose. Plantlet survival, height of the aerial part, and the length of the longest root were significantly greater on half MS medium containing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Carbon source; Micropropagation; Orchid; Mineral salts. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000400001 |
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| Wiebke,Beatriz; Ferreira,Fabricio; Pasquali,Giancarlo; Bodanese-Zanettini,Maria Helena; Droste,Annette. |
| The influence of different antibiotic treatments in soybean genetic transformation was evaluated. First, an assay was performed to verify how different antibiotic treatments affect soybean embryogenic tissues. The effect of carbenicillin at 500 mg L-1 was genotype-dependent. This antibiotic did not affect embryo survival of cv. IAS5, but a three-fold increase of embryo proliferation was observed for cv. Bragg, when compared to the control. On the other hand, cefotaxime at 350 and 500 mg L-1 caused death of embryogenic tissues of both cultivars. Finally, the association of cefotaxime (250 mg L-1) + vancomycin (250 mg L-1) did not affect negatively the somatic embryos of tested cultivars until 63 days of treatment. Thereafter, a second experiment was carried... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agrobacterium tumefaciens; Carbenicillin; Cefotaxime; Genetic transformation; Glycine max; Vancomycin. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052006000400002 |
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| Droste,Annette; Silva,Anelise Machado da; Souza,Iliane Freitas de; Wiebke-Strohm,Beatriz; Bücker-Neto,Lauro; Bencke,Marta; Sauner,Márcia Viviane; Bodanese-Zanettini,Maria Helena. |
| The aim of this work was to identify Brazilian soybean (Glycine max) genotypes with potential to respond to in vitro culture stimuli for primary somatic embryo induction, secondary embryo proliferation and plant regeneration. Differences among eight tested cultivars were observed at each stage. Two cultivars, IAS-5 and BRSMG 68 Vencedora, were selected for the evaluation of the capacity for embryo differentiation and plant regeneration. These cultivars had high embryo induction frequencies, repetitive embryogenic proliferation, and low precocious embryo germination in the initial experiment. The effect of abscisic acid (ABA) and charcoal addition on plant regeneration was investigated. The addition of ABA to proliferation medium and of ABA and activated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Brazilian cultivars; Embryogenic potential; Genetic engineering; Soybean transformation. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700012 |
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| Registros recuperados: 13 | |
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